Subha N Sarcar

Assistant Professor of Instruction; Biological Sciences
Senior Lecturer - Biological Sciences
 
972-883-4128
FO3210

Professional Preparation

Ph.D. - Molecular Cell Biology
The University of Texas at Dallas - 2018
M.S. - Molecular Cell Biology
The University of Texas at Dallas - 2010
M.Sc. - Bioinformatics
Institute of Advanced Studies in Education (IASE), India - 2006
B.Sc. - Biotechnology
Savitribai Phule Pune University (formerly, University of Pune) - 2004

Research Areas

DNA/RNA editing
My research involved, understanding the mechanism of DNA/RNA editing, by single sub unit RNA polymerase family members. We used in vitro techniques to understand the mechanism of unique co-transcriptional RNA editing in Physarum polycephalum, a slime mold from Myxomycete family. 
RNA polymerases
My research involved, understanding the mechanism of DNA/RNA editing, by single sub unit RNA polymerase family members. I have done extensive work on T7 RNA polymerase, as well as SP6 and T3 RNA polymerases. I have also characterized eukaryotic mitochondrial polymerase, with respect to RNA editing. 
Mitochondria related Diseases
Mitochondria is the powerhouse of the cell and is essential for the survival. In human beings, mitochondrial dysfunction can lead to mitochondria related diseases, such as DMD (Duchenne muscular dystrophy) and other age related degenerative diseases such as Parkinson's, Alzheimer's etc. Therefore, it becomes paramount to understand the functioning of mitochondrial system. The mitochondrial RNA polymerase is an essential enzyme involved in the transcription step in central dogma, and has been also implicated to be involved in RNA editing activity in mitochondria. Therefore it is essential, that we study the evolution, mechanism and involvement of mitochondrial RNA polymerase; with respect to the survival and sustenance of the cell, especially if we want to have a background understanding of mitochondria related diseases.  
Cloning Techniques
I have worked on optimizing the Cloning techniques in E.coli, by using homology recombination and used this technique to characterize Physarum mitochondrial RNA polymerase with the help of T7 RNA polymerase.

Publications

Promoter Length Affects the Initiation of T7 RNA Polymerase In Vitro: New Insights into Promoter/Polymerase Co-evolution, Journal of Molecular Evolution, volume 88, pages, 179–193(2020)
2020 - Journal Publications
A specific, promoter-independent activity of T7 RNA polymerase suggests a general model for DNA/RNA editing in single subunit RNA Polymerases, Nature: Scientific Reports, volume 8, Article number: 13885 (2018) 2018 - Journal Publications
Chapter 4 - Genomics and Gene Expression in MyxomycetesMyxomycetes Biology, Systematics, Biogeography, and Ecology, 2017, Pages 107-143 2017 - Book Chapter

Appointments

Assistant Professor of Instruction; Department of Biological Sciences
The University of Texas at Dallas [2019–Present]
Senior Lecturer, Department of Biological Sciences
The University of Texas at Dallas [2018–2019]
Instructor, Department of Biological Sciences
The University of Texas at Dallas [2018–2018]