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Ph.D. - Cell Regulation U.T. Southwestern Medical Center - 1999
B.S. - Biology U.T. San Antonio - 1993
Certificate Optical Lab. Tech. - Optics Ft. Fitzsimmons/Regis College - 1985
M.D. - Medicine Universidad A. de Nuevo Leon Monterrey, Mexico - 1980
Mammalian cell culture and subculture (normal human epithelial cells such as skin keratinocytes, oral keratinocytes, mammary epithelial cells, squamous esophageal cells, and bronchial epithelial cells; normal human stromal cells such as dermal fibroblasts, oral fibroblasts, esophageal fibroblasts, and lung Hbroblasts; human cancer cells such as HeLa, H1299, etc.).
Transient and stable transfections (plasmid transfections with Fugene6 and retroviral plasmid transfections).
Isolation and purification of proteins.
Western blotting, ELISA, and Immunoprecipitation.
Enzyme activity and kinetic assays.
Electrophoretic Mobility Shift Assay (EMSA).
DNA and RNA isolation.
DNA sequencing, gene mapping, restriction digest, and agarose gels.
Northern blotting and RNase Protection Assay.
Plasmid constructs, bacterial competent cell (DH5alpha) culture, cloning and subcloning.
Expressing target gene in bacterial and mammalian cell lines, and site-directmutagenesis.
PCR and Semi-quantitative RT-PCR.
Aging and Cellular Senescence Biology
TRAP (Telomeric Repeat Amplification Protocol) assay, an enzyme assay for detection of telomerase activity.
TRF (Terminal Restriction Fragment) assay, a modified Southern gel to measure telomere length.
Invasive assays in organotypic cultures (3-dimensionaI).
Sato, M., Larsen, J.E., Lee, W., Sun, H., Shames, D.S., Dalvi, M.P., Ramirez, R.D., Tang, H., DiMaio, J.M., Gao, B., Xie, Y., Wistuba, I.I., Gazdar, A.F., Shay, J.W. & Minna, J.D., Human Lung Epithelial Cells Progressed to Malignancy Through Specific Oncogenic Manipulations, Mol. Cancer Res., 11:638-650 2013 - Publication
Garcia-Oscos, F., Salgado, H., Hall, S., Thomas, F., Farmer, G.E., Bermeo, J., Galindo, L.C., Ramirez, R.D., D’Mello, S., Rose-John, S. & Atzori, M., The Stress-Induced Cytokine Interleukin-6 Decreases the Inhibition/Excitation Ratio in the Rat Temporal Cortex via Trans-Signaling, Biol Psychiatry, 71:574-582 2012 - Publication
Vaughan, M.B., Ramirez, R.D., Andrews, C.M., Wright, W.E. & Shay, J.W., H-Ras Expression in Immortalized Keratinocytes Produces an Invasive Epithelium in Cultured Skin Equivalents, PLoS ONE, 4(11):e7908 2009 - Publication
Feagins, L.A., Zhang, H.Y., Hormi-Carver, K., Quinones, M.H., Thomas, D., Zhang, X., Terada, L.S., Spechler, S.J., Ramirez, R.D. & *Souza, R.F., Acid has anti-proliferative effects in nonneoplastic Barrett's epithelial cells, American Journal of Gastroenterology, 102:10-20. 2007 - Publication
Jaiswal, K.R., Morales, C.P., Feagins, L.A., Gandia, K.G., Zhang, X., Zhang, H.Y., Hormi-Carver, K., Shen, Y., Elder, F., Ramirez, R.D., Sarosi Jr., G.A., Spechler, S.J., & *Souza, R.F., Characterization of telomerase immortalized, non-neoplastic, human Barrett's cell line (BAR-T), Diseases of the Esophagus, 20:256-264. 2007 - Publication
Lam, D.C.L.. Girard, L., M., Ramirez, R.D., Chau, W.S., Suen, W.S., Sheridan, S., Tin, V.P.C., Chung, L.P., Wong, M.P., Shay, J.W., Gazdar, A.F., Lam, W.K. & *Minna, J.D., Expression of nicotinic acetylcholine receptor subunit genes in non-small cell lung cancer reveals differences between smokers and non-smokers, Cancer Research, 67:4638-4647. 2007 - Publication
Ramirez, R.D. Overview of Senescence. In David A. Gewirtz, Shawn E. Holt & Steven Grant (Eds.), Apoptosis, Senescence, and Cancer (2 ed., chap. 8, pp. 145-157). Totowa, NJ: Humana Press Inc. 2007 - Publication
*Souza, R.F., Lundsford, T., Ramirez, R.D., Zhang, X., Lee, E., Shen, Y., Owen, C., Shay, J.W., Morales, C. & Spechler, S.J., GERD is associated with shortened telomeres in the sguamous epithelium of the distal esophagus, American Journal of Physiology Gastrointestinal and Liver Physiology, 293: G19-G24. 2007 - Publication
Central Park Campus of Collin County Community College District
Research Instructor The University of Texas Southwestern Medical Center [2015–2020]
Assistant Professor The University of Texas Southwestern Medical Center [2003–2007]
Assistant Professor Dallas VA Medical Center [2003–2007]
Postdoctoral Fellow The University of Texas Southwestern Medical Center [2002–2003]
Postdoctoral Fellow Dallas VA Medical Center [2000–2002]
Graduate Research Associate The University of Texas Southwestern Medical Center [1994–1999]
Research Associate U. T. San Antonio [1993–1994]
Student Intern/Undergraduate Fellow U. T. San Antonio [1990–1993]
Family Physician Private Office Monterrey,Mexico [1982–1984]
Web Publishing Software
Scientific Plotting/Graphing Software
Reference Management Software
Gross Speed: 66 WPM
Net Speed: 64 WPM
Financial Management Software
1988-1992 U.S. Army San Antonio, TX Sergeant
Enrolled as Reserve member of U.S. Army.
Ordered to stand by during The Gulf War (1990).
1984-1988 U.S. Army Ft. Hood, TX Sergeant
Enrolled as Active member of U.S. Army.
Fabricated military frames and lenses.
Executed daily operations of military defense practices, including supervision of medical assistance maneuvers.
Directed and managed three full-time lab technicians in the Optical Laboratory atFt. Hood, TX.
Assisted subordinates and supervisors in identifying and resolving optical related problems.