Ph.D. - Genetics
USSR Academy of Sciences, Moscow, Russia - 1983
M.S. - Plant and Animal Genetics and Cytology
Novosibirsk State University, Russia - 1975
B.S. - Plant and Animal Genetics and Cytology
Novosibirsk State University, Russia - 1973
Borovkova I. G., Steffenson B. J., Jin Y., Rasmussen J. B., Kilian A., Kleinhofs A., Rossnagel B. G., Kao K. N. 1995. Identification of molecular markers linked to the stem rust resistance gene rpg4 in barley. Phytopathology, 85, 181-185. 1995 - Publication
Borovkova I. G., and Loscutov A. V. 1993. Isozymes of sunflower and their use in selection. In: Sunflower (ed. Penchukov V. P.), Nauka, Moscow, pp. 61-64. 1993 - Publication
Bomvkova I. G. 1993. Molecular biology of sunflower proteins. In: Sunflower (ed. Penchukov V. P.), Nauka, Moscow, pp. 65-71. 1993 - Publication
Loscutov A. V., Anisimova 1. N. Borovkova I. G. 1992. Identification of sunflower lines based on electrophoresis of helianthinins and isozymes. Proc. Russian Acad. Agriculture, 6, l2-15. 1992 - Publication
Borovkova I. G., Loscutov A. V., Tolmachev V. V. 1991. Analysis of inheritance and linkage of loci coding morphological traits and isozymes in srmflower. Genetika (Russ.), 27, 1773-1780. 1991 - Publication
Pfizer, St. Louis, MO [2003–2003]
Molecular analyst (Principal investigator)
Monsanto Co., St. Louis, MO [1999–2002]
Monsanto Co., St. Louis, MO [1997–1999]
North Dakota State University, Fargo, ND [1992–1997]
North Dakota State University, Fargo, ND [1991–1992]
University of Texas at Dallas [2003–Present]
BIOL4380 - Molecular and Cell Biology Laboratory
BIOL3301 - Classical and Molecular Biology (summer)
Identification of RAPD markers linked with disease resistance in barley.
1993–1993 Borovkova I. G., Steffenson B. J., Rasmussen J. B., Jin Y., Rossnagel B. G., Kao K. 1993.APS annual meeting, Fargo, North Dakota.
Molecular analyst, Genomics and Biotechnology Group January-July, 2003
Performed site-directed mutagenesis and cloned DNA sequences for fimctional analysis and expression in multiple systems for oncology and osteoporosis studies. Monsanto Co., St. Louis, MO
Molecular analyst (Principal investigator), Regulatory Sciences 1999-2002
Directed molecular characterization of transgenic events for regulatory approval of federal agencies. Implemented research plans in the specific project area. Coordinated work of other team members under pressing timelines. Build putative sequence and restriction map of a near 100 kb genomic region containing a transgene cassette and flanking genomic sequences of a maize transgenic event by using molecular markers, sequence comparison and BAC clones analysis. The generated map was essential for resolving complex regulatory issues and commercial line certification. Contributed to the European approval of Roundup Ready soybean and com lines by successfully resolving molecular structure of complex transgene integration events. Developed a real time PCR assay for high throughput analysis of transgenic lines seed purity evaluation. The assay has been implemented into the SOP for seed quality control.
Molecular geneticist, Disease control 1997-1999
Identified several soybean genes putatively responsible for disease resistance using homology search, EST analysis, transcriptional profiling, and other bio-informatics tools. Initiated and coordinated an interdepartmental effort for mapping, identification and cloning of plant genes involved in pathogen resistance. More efficient resource utilization led to a significant acceleration of a linkage map construction process and successful cloning of a resistance gene. Developed new molecular markers for disease resistant genes in barley for cloning purposes.
North Dakota State University, Fargo, ND
Research Associate, Department of Plant Pathology 1992-1997
Performed mapping of the resistance genes in barley. By persistent use of different molecular markers was the first one to position three resistant genes on the barley map. Received certificate of recognition from the Department for scientific achievements. Managed molecular biology laboratory, supervised and trained technical personnel and graduate students.
Visiting Scientist, Department of Crop and Weed Sciences 1991-1992
Developed molecular markers (RAPD and RFLP) for identification and distinction of sunflower downy mildew (Plasmopara halstedii) races. Improved DNA extraction method for Plasmopara halstedii and was the first one to use RAPD markers for its races identification.
- Molecular biology: Experienced in the most recent molecular biological methods: DNA and RNA isolation; Southern and Northern blot analysis. DNA and protein analysis methods, different types of electrophoresis, including F IGE; gel image analysis. DNA probe construction; molecular cloning and library (bacterial, lambda and cosmid) construction, dot, blot and colony hybridizations; radioactive and nonradioactive DNA labeling and detection; site-directed mutagenesis; Gateway Cloning Technology. 3'- and 5'- RACE, RT-PCR, PCR amplification, product analysis and cloning, genome walking, TAIL PCR, inverse PCR, real-time PCR (TaqMan, Opticon), fluorescent probes. Molecular markers (RFLPs, AF LP, microsatellites, RAPDs).
- Computer skills: Apple, IBM operative systems. Database sequence searching (Incyte, NCBI, national plant genome databases). DNA analysis: GCG Wisconsin Package, Sequencher, DNA Strider; DNAStar, PrimerSelect (trained in sequence analysis at Monsanto internal classes and Monsanto Sequencing Center seminars). Proficient in genetic map constructing software such as MapMaker, .IoinMap, G-Mendel. Microsoft Office 2000 and NT (Word, Excel, PowerPoint).
Creative, goal-oriented professional with demonstrated experience in a variety of molecular biology and genetics techniques. Author of more than twenty publications. Highly organized with the ability to manage multiple projects and meet deadlines. A team player, effectively designing new research projects, troubleshooting and interpreting experimental results. Good communication and presentation skills. Computer literate and trained in DNA analysis bio-informatics tools.